FAP Tagging Vectors
We are developing a set of tagging vectors to make it easy to create your own FAP-fusion proteins. The first available tagging vector is based on Sigma's 3xFLAG vector, and allows for easy creation of an N-Terminal FAP tag. The vector map is shown below.
This vector codes for a FAP that is a tight binder of the se-Red™ series of fluorogenic dyes, as well as binding the ultra bright Dyedrons™.
By itself, the vectors expresses a cytosolic FAP protein that can activate se-Red-s (the cell permeant fluorogen). The figure below shows confocal imaging of lipofectamine transfected HEK cells (excitation 640, emission 680lp). This is a useful control of gene expression prior to creating the fusion utilizing the multi-cloning site.
We bundle 10μg of the plasmid (so you won't need to do a prep right away), along with enough se-Red-s fluorogen to test 5 96 well microtiter plates. Additional dye can be purchased in 1, 10 and 100 plate vials. Volume discounts are available.
The N-Terminal pFAP-L5-CMV plasmid + fluorogen is priced at $500. Contact Us to place an order.
Certain restrictions apply when a Channel Partner is already providing a FAP-based application (GPCR's for example). If you're looking to create a GPCR-FAP fusion, please contact our partner SpectraGenetics, they have a large selection of GPCRs-FAP fusions ready to go, and will create custom fusions on request. FLAG is a registered trademark of Sigma corporation.