- The role of trafficking mutations in lysosomal storage disorders. We're working on assays to search for new drugs against these trafficking mutations. See the discussion regarding Gaucher's disease here.
- We're hiring great biologists! At least we will be if we get your resume (or the resume of one of your extraordinary colleagues). We're building the team that creates the industry's leading cellular trafficking assays, and we're starting with biologists with experience in cell-culture, molecular biology and assay development for high throughput screening. Please check out our careers page, and forward along to qualified candidates. We'll be posting additional positions in the coming weeks. Exciting times!?
- We've just released a new PgP(G268V) mutant trafficking assay.
This assay has been used as selectivity assay when developing CFTR trafficking compounds. For example, Vertex's VX-809 clinical candidate increases CFTR trafficking, but does not also increase PgP trafficking. However, and earlier, less selective compound VRT-325 increases surface expression of CFTR and PgP.
The assay is available as a service, and we can also provide you with cells to run the assay in your own lab. Just let us know...
- hERG Trafficking Assay in Development
We're getting ready to release a new trafficking assay for hERG, for cardiotox testing, and as an important selectivity screen for compounds aimed at correcting trafficking defects (especially for those studying correctors of CFTR mis-folding). Just like CFTR, we'll have wild-type and mutant hERG assays available.
The assays will initially be available as a service for profiling compounds, and as we get assay protocols fully optimized, we'll make cell-lines and assay kits available.
See http://www.sharpedgelabs.com/on-the-bench/herg-trafficking/ for details.?
- Cystic Fibrosis Assays Now Available
We've just moved our CFTR assay from "on the bench" to "off the shelf!"
The assay tracks cell-surface expression of both wild-type and ?F508 CFTR. By using cell-excluded, and cell-permeant dyes we can directly measure both surface and total protein. This allows us to distinguish different CFTR rescue mechanisms (for example, compounds that shift the Surface/ER equilibrium, versus compounds that increase total protein, and thus surface protein, without specifically increasing the fraction at the cell-surface).
We've also included an Application Note, where we test several known CFTR correctors, alone and in synergistic combinations.
The assay is available as a service, or as reagents for more advanced users (we can train in the use of the assay). The assay is running using high-speed flow cytometry.
We're excited to be able to bring this important new assay on-line for our customers. Have a look and contact us if you'd like to test some compounds in the assay.?